I want to test the effects of some compounds on Treg proliferation. So far, I have made a coculture of tumor cells and PBMCs (10x more PBMC), added my compound and looked at %Tregs with flow cytometry after 24h. Do you know what the appropriate time is to look at inhibition of Treg proliferation? Do I have to add anti-CD3?
Thank you
Debanjan Mukhopadhyay
You should stain your cells first with CFSE dye and then add mitogenic stimulation with or without your compound for testing, keep the cells at least for 72 hrs and then measure flow cyrometrically using blue laser of 488 nm excitation for CFSE and 510 filter for emission. If you need I can give you the protocol also.
1 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Mathematical Sciences
- Statistics
- Statistical Software
- SPSS
More Julie Jacobs's questions See All
Similar questions and discussions