What is the cause of streaking and double banding on gels?

Julie Bethany Rakes @Julie-Bethany-Rakes

05 March 2024 1 6K Report

I'm working on library prep for ITS NGS using Earth Microbiome Protocol and am getting double banding and smearing on my gels. What might be the cause for this? I should be seeing a band around 230 bp.

Hari Shankar Gadri

Your products smeared and degraded.

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