What is the cause of streaking and double banding on gels?

Julie Bethany Rakes @Julie-Bethany-Rakes

05 March 2024 1 6K Report

I'm working on library prep for ITS NGS using Earth Microbiome Protocol and am getting double banding and smearing on my gels. What might be the cause for this? I should be seeing a band around 230 bp.

Hari Shankar Gadri

Your products smeared and degraded.

Badges
Science topic

More Julie Bethany Rakes's questions See All

Why would E. coli have higher results than Fecal Coliform results from the same sample?

During recent sampling for E. coli, most of the sample results were significantly larger then the Fecal numbers. What could possible cause this? Method was membrane filtration reporting in CFU.

18 March 2024 7,848 3 View

Studies on oceanography - Plastic pollution of our oceans?

I need help, doing a paper on the plastic pollution of our oceans.

10 January 2024 9,313 1 View

Why does Mendeley keeps adding an 'a' to the end of a citation unnecessarily?

Some of my in-text citations references show up with an 'a' at the end (e.g., Smith, 2023a). The author does not have a 2nd publication in my Mendeley library, and I have checked for duplicates....

14 August 2023 1,436 5 View

Ultrapure water for LC-MS/MS: which is best between Evoqua LaboStar PRO TWF UV and Direct-Q® 3 Milli-Q systems?

Dear all, we are doing LC-MS/MS metabolomics and proteomics (research & diagnostics) and I wondered if you had experience with the Evoqua LaboStar PRO TWF water purification system? It's a bit...

19 July 2023 1,532 5 View

How can I add link to open access punlication?

ResearchGate lists multiple open access publications with the message: "Your recent article doesn’t have the full-text yet. Add the full-text of your recent life science work so that others can...

26 May 2023 7,243 1 View

Is there anyone who could provide an insight on Scopus indexed journals for management and social sciences?

It is mandatory that academicians require publication in Scopus Indexed Journal. It is a little tedious in finding out such resources. What was available last year is not available this year. The...

17 May 2023 3,661 2 View

Black aggragates in ELISA assays, what are those?

Hello, End of last year I have developped an indirect ELISA method to detect a couple of caseins in fermentation supernatants. It worked great until a couple of weeks ago. Since then, for each...

26 April 2023 9,506 2 View

Is thematic analysis part of methodology?

I'm new to thesis work and I am thoroughly confused. I understand what thematic analysis is. My professor put down " Results and Thematic analysis" in the table of contents. But it seems wrong. Is...

25 April 2023 611 5 View

If I want to compare two correlations what's the best way to do that in SPSS?

If I have two correlations: one between variable 1 and variable 2; second between variable 1 and variable 3. What's the best way to analyze the data so that I am able to make the assumption that...

22 April 2023 5,343 4 View

Corning MITO+ Serum extender??

Hello, Does anyone have a substitute for Corning® MITO+ Serum Extender ? Or have the specifications for the compounds in it? It seems to be on back ordered forever. Please help. julie

16 April 2023 2,164 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you know best conditions for formation of best REE deposits?

I want to know more about REE deposits.

03 August 2024 6,930 2 View

Why cycling of matter important to life on Earth & movement of matter through an ecosystem different than movement of energy through an ecosystem?

Why is the cycling of matter important to life on Earth and movement of matter through an ecosystem different than the movement of energy through an ecosystem?

03 August 2024 5,061 3 View

Why does the flow of energy through the biosphere depend on the cycling of nutrients and matter and energy flow across the earth's subsystem?

03 August 2024 6,412 0 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

How do energy flow and matter change in Earth's interior and geosphere subsystem affect the flow of matter and energy on Earth?

02 August 2024 9,144 0 View