Julie Jacobs

3 Questions 3 Answers 0 Followers

Questions related from Julie Jacobs

Plasmid Problems: Is it possible to use RE to cut a section out of a plasmid & immediately amplify it?

I have a 1.6kb insert that has been cloned into a plasmid. I would like to cut a section out of this plasmid that is flanked by restriction sites for ECOR1. Would it be possible to digest the...

12 February 2019 3,034 7 View

Can you detect secreted ATP by luminescence if your supernatans contains FBS?

I've cultured and treated my cells in complete media (containing 10%FBS). Supernatans was collected, centrifuged and stored at -20°C. Now I would like to test ATP release between different...

27 April 2017 3,917 5 View

Does anyone know how to test inhibition of Treg proliferation?

I want to test the effects of some compounds on Treg proliferation. So far, I have made a coculture of tumor cells and PBMCs (10x more PBMC), added my compound and looked at %Tregs with flow...

27 October 2015 407 2 View