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Questions related from Debanjan Mukhopadhyay
Hi, I am growing the SK-MEL-3 cells in DMEM-Hi Glucose with, 10% FBS, 2 mM Glutamine (this is additional to the 2 mM L-gln that is already supplied in the media), Pen-Strep, Gentamicin. But the...
26 October 2024 195 0 View
I am using MEF (From BL/6 mice) as a model. While testing cell death in experiments, I am using MEF with TNF (20 ng/ml), CHX (10 micrograms/ml) as a positive control for apoptosis (TC) and those...
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I wish to perform a cell viability assay using Ubiquitin activating enzyme inhibitor (UBE1). PYR41, the first generation is toxic to the cells of my experiments (human primary foreskin...
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I seeded 1X105 cells in 96 well plates in RPMI 1640+ 10% FBS medium and also with my different stimulus, although I got difference between groups but I saw that even unstimulated cells produce lot...
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What will be ideal passage number range for doing experiments?
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