Debanjan Mukhopadhyay

3 Questions 26 Answers 0 Followers

Questions related from Debanjan Mukhopadhyay

Why in MEF, I am seeing pMLKL after TNF and CHX treatment but not pRIP while I can see the later in TNF+CHX+ZVAD treatment?

I am using MEF (From BL/6 mice) as a model. While testing cell death in experiments, I am using MEF with TNF (20 ng/ml), CHX (10 micrograms/ml) as a positive control for apoptosis (TC) and those...

06 December 2020 6,175 1 View

Can anyone suggest a good inhibitor (non toxic) of Ubiquitin activating enzyme inhibitor (UBE1). I have tried PYR41 but this one is toxic to cells?

I wish to perform a cell viability assay using Ubiquitin activating enzyme inhibitor (UBE1). PYR41, the first generation is toxic to the cells of my experiments (human primary foreskin...

13 August 2018 2,427 5 View

For Raw 264.7 murine macrophage like cell lines can be grown for how many passages?

What will be ideal passage number range for doing experiments?

22 April 2016 2,721 6 View