Debanjan Mukhopadhyay

5 Questions 37 Answers 0 Followers

Questions related from Debanjan Mukhopadhyay

Can anyone suggest about how to grow the SK-MEL-cells in DMEM Hi-Glucose medium?

Hi, I am growing the SK-MEL-3 cells in DMEM-Hi Glucose with, 10% FBS, 2 mM Glutamine (this is additional to the 2 mM L-gln that is already supplied in the media), Pen-Strep, Gentamicin. But the...

26 October 2024 199 0 View

Why in MEF, I am seeing pMLKL after TNF and CHX treatment but not pRIP while I can see the later in TNF+CHX+ZVAD treatment?

I am using MEF (From BL/6 mice) as a model. While testing cell death in experiments, I am using MEF with TNF (20 ng/ml), CHX (10 micrograms/ml) as a positive control for apoptosis (TC) and those...

06 December 2020 6,217 1 View

Can anyone suggest a good inhibitor (non toxic) of Ubiquitin activating enzyme inhibitor (UBE1). I have tried PYR41 but this one is toxic to cells?

I wish to perform a cell viability assay using Ubiquitin activating enzyme inhibitor (UBE1). PYR41, the first generation is toxic to the cells of my experiments (human primary foreskin...

13 August 2018 2,472 5 View

What might be the reasons If I get high levels of IL12 and TNF but low levels of IFNg in culture supernatants of unstimulated PBMC ?

I seeded 1X105 cells in 96 well plates in RPMI 1640+ 10% FBS medium and also with my different stimulus, although I got difference between groups but I saw that even unstimulated cells produce lot...

30 December 2016 5,531 4 View

For Raw 264.7 murine macrophage like cell lines can be grown for how many passages?

What will be ideal passage number range for doing experiments?

22 April 2016 2,779 6 View