Elmira Zarei

9 Questions 6 Answers 0 Followers

Questions related from Elmira Zarei

How should I make a positive control of autophagy by NH4CL?Which concentration is good for nalm?

Hi everyone ,does any one know how can I make a positive control for autophagy pathway with NH4Cl? I’m working on nalm6 cell line

29 May 2022 9,791 0 View

Does anyone know how to remove primer dimer in standard curve of Real time?

Hi everyone ,I am working on three autophagy genes I want to calculate the efficacy so I dilute my samples in this way:1/10,1/100,1/1000 and so on.But the dilutions more than 1/1000 have primer...

20 April 2022 5,388 1 View

Why the result of one drug in mtt assay on pbmc are the same?

Hi researchers! I have gained something strange in the result of mtt assay of the PBMC. I was testing an herbal extract that is declared to be safe for normal blood cells by researchers but I saw...

14 April 2022 5,360 2 View

How to eliminate primer dimer in RTqpcr?

Hi researchers I am doing real time for some autophagy genes . At first some of them didn‘t have any bonds or had primer dimer!! So I have reduced primer concentration ,increased the template...

05 April 2022 6,476 3 View

How to set up a MTT assay for PBMC?

Hi researchers! I want to run a mtt assay on PBMC to show that a cytotoxic drug in a leukemia, does not have any effect on normal cells I mean PBMC! Is there any special protocol to isolate ,seed...

03 April 2022 3,672 3 View

Why necrosis is seen instead of apoptosis?

Hi researchers, I was searching for the synergism of two different drugs by running mtt assay,I analyzed my data and I found them synergism, so I used each drug IC50 and both Ic50 to check them by...

18 December 2021 6,360 3 View

How to analyze synergism effect of 2 drugs in mtt assay by graphpad prism?

Hi researchers I need your help again. Any one can tell me how to analyze synergism effect in graphpad prism? My data is the absorbance of each well, I used the IC50, IC50/2 and IC50/4 and 2IC50...

21 September 2021 4,261 4 View

How sould I manage synergism effect of two drugs in MTT assay?

Hi, Dear researchers I need your help. I'm working on 2 drugs to find IC50 of them in nalm6 cell line, I found each IC50 separately but I wanna find their synergism effect and their combination...

04 September 2021 7,136 4 View

Why my formazan crystals isn't solved in my mtt after addition dmso and even shaking 1 hour??

Hi researchers I have a problem I'm doing a MTT assay on a ALL cell line. of course this cell line is suspended so I used u form 96 well Plates at first... I seeded 20000 cells in 96 well plates...

05 July 2021 1,312 5 View