PI3K class II alpha inhibitor?

More Aurelia E Lewis's questions See All

Size-biased recovery of total RNA isolated with Trizol vs RNeasy?

So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...

14 July 2024 641 1 View

Is it meaningful to take mean averages of scores that range from 1 - 3?

Hi, if someone has data that are scored: 1 for poor, 2 for adequate and 3 for good. Can you take a mean average if a score of less than 1 is not possible? I'm thinking if a mean score of...

23 April 2024 6,366 1 View

Is there a single database on body mass and/or height in a diversity of adult human populations?

I'm keen to view data on the body masses of people (and/or heights), disaggregated by sex, representing a good diversity of human populations, based on large sample sizes and including a measure...

16 April 2024 10,088 1 View

Should I be calculating foliar Zinc fertilizer according to the height of the pot (29cm) or to the depth at which soil was collected?

Please in an experiment using foliar Zinc fertilizer and rice I obtained soil from a depth of 20cm and placed 5kg of the soil in pots of 29cm in height, diameter 32.5 cm, radius 16.25 cm and...

02 July 2023 9,616 1 View

How should I be dissociating 30k embryonic epithelial cells for FACS to prevent clumping?

The project I'm working on involves dissecting out a small piece of epithelial tissue that we're going to run through FACS and single cell RNA seq. My usual way of dissociating tissue is in 50ul...

24 June 2023 9,688 0 View

Can T cells de-differentiiate?

Hi, Does anybody know whether effector or memory T cells can de-differentiate into naive T cells, rather than just become exhausted or undergo apoptosis? Thanks

19 March 2023 4,057 2 View

How to interpret negative values from analysis of soil NO3 using KCl extraction+a continuous flow analyser?

I used the KCl extraction method to extract nitrate from soil samples which were then analysed using a skalar continuous flow analyser. I am doing this to calculate the rate of nitrification from...

09 February 2023 9,959 5 View

Availability of SSQ-12 in Turkish?

Hi, I would like to conduct a study using the Turkish version of the short form Speech, Spatial, and Qualities of Hearing Scale - does anybody have a copy? Best wishes, Aaran

22 January 2023 9,946 3 View

Anybody know what PD-1-negative CD8+ T cells are doing in tumours?

I understand the role of activated/exhausted CD8+ T cells expressing PD-1 in tumours but just wondered if anybody knew of any functions attributed to CD8+ T cells that don't express PD-1?

17 January 2023 2,246 0 View

In MATLAB simulink I connected a 3-phase series RLC load to a Induction motor to study the starting characteristics but following error is coming?

The following two blocks cannot be connected in series: Block 1: 'A_stator: Asynchronous Machine pu Units' Block 2: 'phase_A: Three-Phase Series RLC Load' The first block, modeled as a...

08 November 2022 7,683 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View