Anoxic protein purification - Impact of sonication on Fe-S cluster proteins?

Dear all, I need to purify anoxically a protein with Fe-S clusters (sensitive to oxygen). I used to do cell lysis using a French press but because the instrument is too big to be place in my...

08 November 2020 9,528 3 View

How to compute the effects in half fractions?

Greetings, I'm doing a screening design and want to allow for lack of fit. I need replications so I want to add four additional points to my dataset. Rather than use center runs I use corner...

11 October 2020 9,672 1 View

How to choose an ID variable in the HLM software student version ?

When I have to choose my identification variable in lvl 1 and lvl 2, I cannot choose it because it is grayed out. Do you know why ? and how to fix it?

27 March 2020 8,558 2 View

Meta-analysis in Stata : how to perform it with effect sizes ?

19 December 2019 9,553 2 View

How to find 3 initial parameters for gompertz curve in presence of other predictors

Given the following when fitting a three parameter gompertz curve to a predictor xg(x) = alpha * exp(- gamma * exp(- beta *x) ) I need to know whether gamma is equal to the inflection point of...

13 May 2017 2,803 11 View

How to find initial parms estimates when doing nonlinear analysis?

proc NLIN data = eleven BEST = 10 MAXITER = 100 METHOD = Gauss CONVERGE=1.0E-6 LIST ALPHA = 0.05 OUTEST = outest(where = (_TYPE_ = "COVB")); parms B1 = 1 B2 = .5128 B3=-.0036; model y =...

26 March 2017 2,174 5 View

What is the best housekeeping gene for a Western blot on purified adipocytes? GAPDH disappears after the purification.

Western blot

19 December 2012 6,810 5 View

Are the hyperspectral image datasets which are publicly available at ehu.eus website atmospherically and geometrically correct?

I've come across a couple of publicly available hyperspectral image datasets from the website http://www.ehu.eus/ccwintco/index.php/Hyperspectral_Remote_Sensing_Scenes Now, my concern is...

05 February 2021 3,550 1 View

How commonly do you encounter Rummination Syndrome in your GI practice?

Do diaphragmatic breathing and speech therapy actually help? How often did you require multidisciplinary team approach?

17 January 2021 5,998 0 View

How to download high quality DEM (resolution less than 5meter)?

I want high-quality DEM for our research purpose. High-quality DEM is used as a reference. I have generated the DEM through stereo images.

27 December 2020 8,614 15 View

Which are the post harvesting processes of turmeric which results in maximum loss of curcumin content of Turmeric?

I am talking about the GI tagged Kandhamal Haladi (Turmeric), which is famous for its curcumin content.There are few blocks in Kandhamal district such as Balliguda where curcumin content is very...

18 November 2020 2,304 3 View

Hello everyone, does anyone have this attached map as a shapefile?

- I did georeferencing and digitizing for it, but it will be more accurate to have the original one.

19 October 2020 9,550 7 View

Why all the consumers are preferred low Glycemic index (GI) Rice for consumption purpose?

Recent days everyone is asking about the Rice varieties which are having low GI are good for health. What are the real health benefits of the low GI Rice varieties ....

15 September 2020 8,495 6 View

Systematic review with studies with the same intervention but two different comparators?

Would it be possible to conduct a systematic review with RCTs that have the same Population, the same intervention (Low GI diet) but some studies compare the Low GI diet to the usual diet and...

06 August 2020 2,170 4 View

How to produce and use multi-resolution grid (a mixture of high and coarse resolution on the same raster image) in ArcGIS or any GIS software ?

It seems impossible since most of analyses are based on 3x3 cell neighborhood but I would like to hear your experiences. I know that some hydrodinamical models like TELEMAC are able to perform...

18 May 2020 6,177 7 View

Is there any algorithm or open source code available for Sentinel 2 bands resampling to 10M in python?

I am doing some agricultural Crop Classification using Sentinel-2 images for that i need to do bands resampling to 10M . I am doing it python, is the any algorithms available for resampling bands...

11 May 2020 8,841 2 View

Is there a method to average multiple hot spot analyses?

We collected three ecological variables from a study area in nearly every months for three years. We would like to perform optimized hot spot analyses in ArcGIS on these months and then check the...

25 April 2020 9,942 4 View