Does anyone know how much DNA is in a Schistosoma egg?

More Javier Gandasegui's questions See All

It is possible to obtaing high-quality DNA from urine samples preserved with forlmol?

Hello, I want to know if it is possible to obtaing high-quality DNA to amplify schistosome-DNA in urine samples previosly preserved with formol 1%. Thanks

07 August 2017 9,741 1 View

Is it possible to diagnose STH and schistosome infections by microscopy in forzen samples of feces?

Hello, I want to know if STH and schistosome eggs are resistant to freezing and can be detected a second time if forzen feces are reanalyzed by the Ritchie concentration method and Kato-Katz. Thanks.

07 August 2017 4,023 11 View

What is the best method to validate molecular or any diagnostc test?

Hello; I am trying to validate or asses the diagnostic reliability of a new test. I have doubt about the necessary number of samples, the comparison between the different diagnostic test employed...

31 December 2015 4,896 4 View

Waht are the best methods for sample collection and storage for DNA extraction?

Hello! We are preparing an study for molecular diagnosis in different type of spicemen: blood, feces and urine. The samples are collected in a different country and we want to avoid the cold...

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What is the best method for DNA extraction from a filter?

Hello, My goal is to filter water with different biological forms and make a DNA extraction from the filter. The technique must be simple and cheap, so I'd like to use a paper filter but I could...

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What bioinformatics parameters should be used to justify the specificity?

I am developing a molecular diagnostic method and I would like to justify the specificity using bioinformatics tools like BLAST or Clustal, as I have no access to DNA from different species. What...

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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fatal error

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fatal error

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

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How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

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Hugo Luca Abate

One way for you to discover it in my opinion, is extract it with a DNA extraction kit and quantify with a technique that you prefer. An example is NanoDrop, but you have others to choose. I hope that i can be helpful.

Lionel Zenner

I will add to Hugo answer that DNA quantity in parasites (or not) eggs varies during time and development of eggs. You will have big differences between first stages and eggs containing a blastula or a morula.