Hello! We are preparing an study for molecular diagnosis in different type of spicemen: blood, feces and urine. The samples are collected in a different country and we want to avoid the cold chain. Can Anyone sugest any protocol or literature for this purpose?
Thanks
For the liquid samples the this might be an option:http://www.luminexcorp.com/publications/index.php?view=single&id=22419
I have not used it myself yet, but I hear good stories about it.
Good luck
Thanks Belgees! It was useful for me. But now I have a nex question, before DNA extraction in feces fixed with EtOH, is it necessary a new previous step?
I use the spin-column method for DNA extraction. Reagents are commercially availabe e.g Qiagen kit. I store my samples at between -20oC--70oC.
for preservation many folks use RNALater, but realistically for DNA work I'd say you dont need it. freezing is optimal, or at least 4C, or for some sample types (eg stool) RT for a few days is OK. tough to isolate DNA from all these samples using one kit. purelink microbiome purification kit is great for feces and urine, but not blood. MagMax total nucleic acid purification kit can probably do all of them, in plate format
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