Hello,
My goal is to filter water with different biological forms and make a DNA extraction from the filter. The technique must be simple and cheap, so I'd like to use a paper filter but I could use a different one as long as it has a pore size of 15-25 um. Anyone know what the best method is?
Thanks.
Dear Javier
Please read the chapter below. It has the protocol for DNA extraction from dried blood spots on filter papers.
Best Regards
Thanks for your answers. However, my problem is that I have to use a filter of 90 mm diameter and for DNA extraction I have to use the whole filter. Any idea?
cut the filter and put it in a 50 ml falcon tube, them extract the DNA.
Are you working with cellulose based filters? then you can dissolve the filter in acetone 80%, in a tube and then allow to evaporate acetone. If this solution is interesting for you, contact with me, I have a device for doing this work.
best
Hi,
the question has been posted some time ago, but I would like to use the same method. DNA extraction from groundwater samples, 1 L water will be filtered with 0,22 um cellulose-acetate filter.
Can i store the filters in a 50 mL tube at -20 C until further processing? Or is it necessary to add something?
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