Does anyone know how I can remove Dnak protein contamination for the sample?

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Dominique Liger

Hi there,

Add ATP to your prep in order to encourage ADP/ATP exchange and then lower DnaK affinity which should allow the release of your protein.

For more details :

http://molbiol.ru/forums/index.php?act=Attach&type=post&id=105788

Hadi H Mohammad

thanks Dominique for your help

Jaime Guillén

Sometimes a washing step with high salt concentration remove these kind of proteins in the protein purification. Another oprion it's add some additional step in the purification if you don't have done yet, exchange ion column, gel filtration column, etc.