Hadi H Mohammad

6 Questions 13 Answers 0 Followers

Questions related from Hadi H Mohammad

Repeated inserts ligated to my plasmid??

Hi all I ligated my small insert (37bp) into 3.5kb plasmid, I got colonies but when I check the sequence of my insert, I found more than one insert ligated as a repeats, some clone got three...

06 February 2019 7,604 6 View

Why the antibiotic test showing different result by disc diffusion test compare to minimal inhibitory concentration?

I got a modified antibiotic , I checked the antibacterial activity by two different assays, first using disc diffusion test which showed less active than the WT. second, by minimal inhibitory...

19 April 2016 3,620 12 View

Does anyone know how I can remove Dnak protein contamination for the sample?

I have purified my protein sample, but still there is a secondary band which is around 70kD, and I need a protocol to remove it from the interest protein. Thanks

06 March 2015 4,053 3 View

Please can you tell me how can I add charge +3 to Fe ion using AutoDock tool or any relevant program?

I am using autodock tool to dock a ligand to a receptor which has Fe ion, but when I run the program I have got a message telling me that Fe ion has zero charge which may affect the docking...

16 February 2015 4,702 8 View

How can I detect and measure the pyrophosphate PPi releasing from enzymatic reaction?

I have purified a protein which suppose to adenylate amino acid consuming ATP and releasing AMP and PPi, so to test the protein activity I have to measure the released PPi in the solution. Can...

14 September 2014 8,560 17 View

Can someone help with flag-tag to the C-terminus of the protein?

Does anyone know how to add Flag-tag to the C-terminus of the protein? What is the principle of the procedure? I am planning to transform bacterial gene to mammalian cells and I have to check the...

26 January 2014 7,792 5 View