Does anyone know good primers for Cryptosporidium meuitable fleagridis- for real-time PCR?

More Małgorzata Raś-Noryńska's questions See All

How to convert IU/ml to ug/ml in case of anti-TSHR antibodies?

Does anybody know how to convert IU/ml in case of anti-TSHR antibodies (Graves disease)? I would like to know how much anti-TSHR Ab I have in a sample

02 February 2024 1,625 3 View

Dear All, do you have any idea regarding dating methods of beach rocks (I mean cement from a carbonate conglomerate, age: last 100 years)?

I expected age between present up to 100 years. I heard U-Th is useless in this case. If you have any idea let me know, please.

27 January 2024 8,273 7 View

How to choose antibodies for western blot?

What are the rules when choosing antibodies

04 September 2023 4,832 4 View

Where can I find examples of practical use of randomly distributed natural fibers to improving the soil in situ, no in the laboratory tests?

I am looking for examples of practical use of randomly distributed natural fibers to improving the soil, e.g. under the foundation or as the top layer of a dirt road, or other. In the literature,...

22 June 2023 340 0 View

Thermal degradation of polystyrene using QMS system: what does m/z=39 come from?

Hello everyone, I would like to ask about thermal decomposition of polystyrene using TG-QMS system - thermogravimetry coupled to quadrupole mass spectrometer. During thermal degradation of pure...

14 February 2023 8,556 3 View

What institutions in Germany monitors the conditions under which a cosnumer may intervene in current environment?

Specifically, I mean how is it working, for example when someone that builds a house, wants to change colours, proportions, ect, things that are basically prescribe on the legislative level. Is it...

23 November 2022 5,448 2 View

What is the best anti-CD8 antibody for IF of paraffin sections of human cancer?

Dear Fellow Researchers, Can you recommend a suitable antibody recognizing human CD8 in paraffin sections of cancer samples? We want to combine it with other markers and do an analysis by...

09 October 2022 3,676 1 View

How to use continuous variables in COX regression?

Dear Fellow Researchers, We have analyzed the effect of various parameters on bladder cancer recurrence. Among included parameters, we had various continuous parameters. In the literature,...

20 August 2022 6,514 4 View

Can I use dialysis bags instead of tubes for Liposomes release profile?

I want to do liposomes release profile, I was wondering if I can use dialysis bags as I already have them cut of 12 kda instead of using dialysis tubes as I have seen everyone is using that.

27 July 2022 3,317 6 View

What are the suspended minerals paricles in seawater?

I am interested in the minerals particles suspended in seawater. What are the minerals? Carbonates, silicates, what more? Which clays are the most common? Could someone provide articles related to...

19 August 2021 6,542 6 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View