I need good primers, that are 100% specific to Cryptosporidium meleagridis and suitable for real- time PCR reaction.
And, possibly, also C meleagridis positiv DNA sample.
hope it will help you http://jmm.sgmjournals.org/content/55/9/1217.full
Likewise, I have used PrimerQuest for the same thing and it gives you good results for primer design.
http://www.idtdna.com/Primerquest/Home/Index
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