hi , i'm doing my experiment with B cells from MD4 mouse. (which has transgenic BCR specific for antigen HEL ) i tried to activate these B cells with VLP expressing HEL antigen on its surface but it doesn't work well and it seems that B cells didn't proliferate well ..after 3 days culture , i checked viability and it was merely over 0.4% .
i read paper saying that VLP works efficiently on B2 cells but not on B1 cells so i wonder ratio of B1 cells and B2 cells in mouse spleen.
+ i also wonder optimal media condition for culturing mouse B cell .
the media i used to culture is RPMI 1640 with 10% FBS , 1% P/S (penicillin & streptomycin) , 1% glutamax
thanks .
Dear Yoorha Kang
Pls. find the attached files and the references, good luck
Hoping this will be helpful to your project
Regards
Prof. Houda Kawas
http://www.pnas.org/content/106/14/5773.full.pdf
http://www.jimmunol.org/content/jimmunol/early/2016/02/04/jimmunol.1403246.full.pdf
http://www.nature.com/ni/journal/v8/n7/full/ni1480.html?foxtrotcallback=true
https://books.google.com/books?id=GKDt-d-TGcMC&pg=PA527&lpg=PA527&dq=ratio+of+B1+cells+and+B2+cells+in+mouse+spleen&source=bl&ots=OZbhoVWz5j&sig=kgShvL5W2jY5EWWMBod-56y2LLY&hl=ar&sa=X&ved=0ahUKEwijk46wpNTVAhViQJoKHaWBCWA4ChDoAQgrMAE#v=onepage&q=ratio%20of%20B1%20cells%20and%20B2%20cells%20in%20mouse%20spleen&f=false
Also
http://www.sciencedirect.com/science/article/pii/S1074761301001297
http://www.sciencedirect.com/science/article/pii/S0092867407009580
http://www.bloodjournal.org/content/bloodjournal/109/11/4627.full.pdf?sso-checked=true
http://science.sciencemag.org/content/293/5537/2111
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118139/
And
Good luck
http://jvi.asm.org/content/75/12/5482.full
http://pubmedcentralcanada.ca/pmcc/articles/PMC4779667/
http://jem.rupress.org/content/jem/193/12/1393.full.pdf
http://onlinelibrary.wiley.com/doi/10.1002/eji.200424819/pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832833/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215630/
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