How to calculate visual angle of a visual stimulus?

I am currently trying to figure out how to calculate the visual angle of some visual stimuli I used in an experiment. I think I have all of the information I need to calculate this. One is a...

15 February 2021 3,189 2 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

How to make sure self-ligation of a linear plasmid?

I want to ligate a linear 1.7kbp plasmid with the EcoRI sites at two sides, but when I use the quick ligase kit from NEB to test the ligation efficiency it is hard to ligate it. Is there anyone...

08 December 2020 3,442 3 View

What effect does light have on the SLS plant tissue decelluarization process?

does it speed it up?

29 November 2020 6,168 3 View

Significant degradation of RNA in RNAlater storage at 4C for longer than a month?

I misread the amount of time that RNA was stable in RNA later at 4 C as several months instead of a month. I have e zebrafish samples that have taken me a 2 months to collect and they have been...

23 October 2020 8,085 3 View

How to present the results of a binary logistic regression for an ecological paper?

I conducted a binary logistic regression using the glm function in R. My binary predictor is the presence or absence of my study species. I have two significant variables but am just not sure what...

19 October 2020 5,398 3 View

Basic question - how to make a cell dissociation buffer?

Dear All, I'm looking to make a cell dissociation buffer from lyophilised collagen I (Gibco, 12648010). The protocols I've been looking at all specify concentrations of enzymes in terms of...

06 October 2020 506 3 View

What is the best way to perform a serial dilution of E.coli, when the culture is starting on a solid media (LB)?

I have an LB growth media plate with E.coli on it. I want to dilute the culture. I am working with 3M Coliform detection plates, and I want to get a solution of E.coli diluted enough to grow...

27 August 2020 3,043 3 View

Why fluorescence signal drops during thermal shift assay?

I am performing a TSA to investigate binding of a ligand to the protein by performing a serial dilution of ligand against same conc. of protein (~2uM). Interestingly at low ligand conc the...

02 August 2020 3,167 1 View

Writing specific contact surface behaviour using Userinter.F in ANSYS?

Hello, Is there anybody who has experience developing any user subroutine using Userinter.F for contact simulations in ANSYS? Any advise and example would be appreciated highly. thanks a lot

21 February 2020 6,495 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View