I am using Ez-Tn5 transposns system to create random mutations in E.Coli. After all the screening procedures I have narrowed down the samples I want to proceed for sequencing. However, I have problems in getting a proper sequence. I have tried inverse PCR methods and now I am following the method in the following paper.

Is there any other working and efficient method to identify the transposons inserted sites?

http://www.epibio.com/docs/default-source/forum-archive/forum-09-2---rapid-identification-of-ez-tn-transposon-insertion-sites-in-the-genome-of-neisseria-gonorrhoeae.pdf

Thank you

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