Prasangi Rajapaksha

2 Questions 4 Answers 0 Followers

Questions related from Prasangi Rajapaksha

Does anyone know an efficient method to identify/sequence transposons insertion sites?

I am using Ez-Tn5 transposns system to create random mutations in E.Coli. After all the screening procedures I have narrowed down the samples I want to proceed for sequencing. However, I have...

31 March 2019 4,933 4 View

Is there a protocol to dialyses the lipidated AcrA protein using PBS? or with low concentration of Triton-X?

The AcrA protein has purified from the E.Coli using a buffer containing Tris-Hcl, NaCl, PMSF, Triton-X and imidazole

22 February 2017 7,941 2 View