Does anyone know an efficient method to identify/sequence transposons insertion sites?

I am using Ez-Tn5 transposns system to create random mutations in E.Coli. After all the screening procedures I have narrowed down the samples I want to proceed for sequencing. However, I have...

30 March 2019 4,819 4 View

The crystallite size from XRD result of thin sheet under Scherrer's formula and thickness of the thin sheet should be same?

I made Indium Tin Oxide(ITO) thin layer and calculated crystallite size by using Scherrer's formula for XRD results. And I measured film thickness by using alpha step equipment and that value was...

21 August 2016 5,431 6 View

Is it possible to use Scherrer's formula to calculate crystallite size of spin coated thin film?

Is Scherrer's formula applicable to use for thin film X-RD results? If anybody knows or if somebody has theoretical details regarding physical relationship between the formula and crystal...

07 August 2016 6,625 5 View

What is happening when we anneal a spin coated ITO on glass substrate in a vacuum atmospheres up to 600C ?

In my ITO sol contains PVA as a binder/stabilizer. I did annealing and I got brown colored layer instead of transparent layer (After annealing my samples were not opaque but it was not good...

27 June 2016 9,676 7 View

What are the indicator species to have idea on climate change?

I think some of the lower plant species (fens, moss, etc...) can use as indicator species, but more infomations are not available for prove that idea.

23 December 2011 3,614 16 View

How to validate and refine Protein structure (PDB cystalography data) ?

I am planing to do a docking analysis of protein 3VW6 using autodock. how can i validate and refine the crystallography structure ? I found these two web site claming to do so. can these sites...

01 January 1970 6,078 2 View

Why do i get a convergence error in gaussina DFT calculations eventhough the stationary state is found during geometry opt ?

Dear sir/madam, what is the reason for the above mentioned observation ? How can I eliminate it ? Thank you

01 January 1970 4,897 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View