http://pic.mbu.iisc.ernet.in/

Harindu Rajapaksha

by downloading the protein and opening it with Biovia Discovery studio I checked the mentioned active site residues and here are they:

HIS374 HIS 378 GLU 402 OR (GLN340 GLU57 THR55 ASN53) OR (LYS26 ASN90) or (ASN322 MET323) or (PHE338 PHE342 PHE343).

you also can check the actual active site residues from Uniprot.

some researchers consider the native ligands that comes with the crystal structure position is the active site.

or you can use online servers for predicting it for you (which I don't prefer) such as:

http://www.pool.neu.edu/wPOOL/help.jsp

http://www.sbg.bio.ic.ac.uk/3dligandsite/ http://consurf.tau.ac.il/2016/

http://cssb.biology.gatech.edu/findsite

http://sts.bioe.uic.edu/castp/

http://www.scfbio-iitd.res.in/dock/ActiveSite.jsp

best regards

http://cssb.biology.gatech.edu/findsite

I am sending you a picture from Lan et al (2020) with all contact residues in ACE2 and Sars-Cov-2 interface.

Source: https://www.nature.com/articles/s41586-020-2180-5

According to CARd, there seems to be amino acids 158 and 570 are responsible for association of spike one. All the...