The yeast (Saccharomyces cerevisae) encodes several homologues of MAPK signaling cascade that play key functions in mating and adaptation to stressful conditions.
I would like to know if these yeast MAPKs are sensitive to pharmacological inhibitors used to study MAPKs in mammalian cells, such as U0126 and PD98059 (MEK1/2), JNK inhibitor VIII, and SB203580 (p38).
It has been reported that SB203580 inhibits Hog1 (yeast p38) in vitro.
Has anyone investigated it? Any reference?
Thank you!
With the exception of the p38 MAPK inhibitors (SB 203580 family), inhibitors to Erk and JNK are fairly poor in potency and specificity. Yeast do not have an orthologue to JNK/SAPK so JNK inhibitors (or Mkk4/7 inhibitors) will not be useful. Fus3/Kss1 are fairly highly conserved with Erk1/2 and Hog1 is well conserved with respect p38MAPK. The MEK orthologue (Ste7) is a bit more distant from Mek1/2 and I couldn't find data on whether those drugs hit the yeast protein. Nice thing about yeast is relative ease of detention strains which are **much** more selective than small molecule inhibitors.
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