5 Questions 11 Answers 0 Followers
Questions related from Jonas Albarnaz
My aim is to detect nascent protein synthesis by click-chemistry of L-homopropargyl-glycine-labelled proteins with biotin azide and detection by western blotting with fluorescently-conjugated...
14 February 2023 8,918 2 View
I would like to know if GM130-positive vesicles can be observed in the cytoplasm far from the Golgi complex.
17 April 2016 8,164 1 View
The yeast (Saccharomyces cerevisae) encodes several homologues of MAPK signaling cascade that play key functions in mating and adaptation to stressful conditions. I would like to know if these...
27 May 2015 179 1 View
Hey everyone, I've had some troubles with my protocol of nuclear fractionation and this forum was recommended. The protocol is based on hypotonic cell lysis with RBS followed by Nonidet P-40...
11 April 2011 1,141 6 View
Hey everyone, I've had some problems with my protocol of nuclear fractionation and this forum was recommended. The protocol is based on hypotonic cell lysis with RBS followed by Nonidet P-40...
11 April 2011 6,025 1 View