Jonas Albarnaz

5 Questions 11 Answers 0 Followers

Questions related from Jonas Albarnaz

Which is the best biotin-azide substrate for click-chemistry to detect nascent protein synthesis with L-homopropargyl-glycine metabolic labelling?

My aim is to detect nascent protein synthesis by click-chemistry of L-homopropargyl-glycine-labelled proteins with biotin azide and detection by western blotting with fluorescently-conjugated...

14 February 2023 8,919 2 View

Does anti-GM130 antibody stain any other subcellular structure other than Golgi?

I would like to know if GM130-positive vesicles can be observed in the cytoplasm far from the Golgi complex.

17 April 2016 8,166 1 View

Does anyone know about sensitivity of yeast MAPK homologues to inhibitors of mammalian MAPKs, such as MEK, p38 and JNK?

The yeast (Saccharomyces cerevisae) encodes several homologues of MAPK signaling cascade that play key functions in mating and adaptation to stressful conditions. I would like to know if these...

27 May 2015 181 1 View

Nuclear Factionation

Hey everyone, I've had some troubles with my protocol of nuclear fractionation and this forum was recommended. The protocol is based on hypotonic cell lysis with RBS followed by Nonidet P-40...

11 April 2011 1,142 6 View

Nuclear Factionation

Hey everyone, I've had some problems with my protocol of nuclear fractionation and this forum was recommended. The protocol is based on hypotonic cell lysis with RBS followed by Nonidet P-40...

11 April 2011 6,027 1 View