How to prevent reoxygenation of cells after incubation in hypoxic chanbers?

We are using hypoxia chambers to induce physiological hypoxia in cell cultural environment. the major problem is, reoxygenation during the cell harvesting, cell lysis, etc. this is the chamber we...

05 June 2016 1,455 5 View

What are your thoughts on immunoprecipitation using total proteins and their phosphorylated form?

I need to analyse the serine phosphorylation elevation and inhibition of STAT5 signalling molecules. So for analyzing the serine phosphorylation level, I have to use anti-phosphoserine antibody....

11 December 2013 1,021 10 View

How to find out the best suitable drug concentration and treatment time?

In my study, on anticancer activity of a drug, I found that IC50 dosage for 2 cell lines are different. In one of my cell lines, the concentration is 60 uM for 24 h and for the other it is 60 uM...

04 May 2013 9,145 5 View

Cell numbers for Xenograft tumor model induction

I am currently working with tumor xenograft models in balb/c athymic mice, and I want to know if the number of cells injected to induce xenograft has any significant role in tumorigenesis. Fewer...

05 June 2012 1,379 36 View

Polymorphism studies

I want to study the ACE gene polymorphism in horse, but i am not able to find any primer informations related to that. Can anyone help me in detecting the polymorphism, i am trying to find out...

08 September 2011 7,057 2 View

Drug dosage

The IC50 dosage of a trial drug is found as 300mM in the invitro analysis (Breast cancer cell lines). they got better result on all the remaining procedure with this concentration. this research...

05 June 2011 4,553 9 View

Targeted Drug Therapy

Each Cellular process have more than one signal pathway connecting to it. So upto what percentage targeted drug therapy is successfull? If we Block one target, will this tumors bypass the blocked...

03 April 2011 6,552 5 View

Targeted Drug Therapy

Each Cellular process have more than one signal pathway connecting it it. So upto what percentage targeted drug therapy is successfull? If we Block one target, will this tumors bypass the blocked...

03 April 2011 7,760 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View