Horse skeletal muscle culture
We are using hypoxia chambers to induce physiological hypoxia in cell cultural environment. the major problem is, reoxygenation during the cell harvesting, cell lysis, etc. this is the chamber we...
05 June 2016 1,597 5 View
I need to analyse the serine phosphorylation elevation and inhibition of STAT5 signalling molecules. So for analyzing the serine phosphorylation level, I have to use anti-phosphoserine antibody....
11 December 2013 1,101 10 View
In my study, on anticancer activity of a drug, I found that IC50 dosage for 2 cell lines are different. In one of my cell lines, the concentration is 60 uM for 24 h and for the other it is 60 uM...
04 May 2013 9,223 5 View
I am currently working with tumor xenograft models in balb/c athymic mice, and I want to know if the number of cells injected to induce xenograft has any significant role in tumorigenesis. Fewer...
05 June 2012 1,661 36 View
I want to study the ACE gene polymorphism in horse, but i am not able to find any primer informations related to that. Can anyone help me in detecting the polymorphism, i am trying to find out...
08 September 2011 7,142 2 View
The IC50 dosage of a trial drug is found as 300mM in the invitro analysis (Breast cancer cell lines). they got better result on all the remaining procedure with this concentration. this research...
05 June 2011 4,653 9 View
Each Cellular process have more than one signal pathway connecting to it. So upto what percentage targeted drug therapy is successfull? If we Block one target, will this tumors bypass the blocked...
03 April 2011 6,629 5 View
Each Cellular process have more than one signal pathway connecting it it. So upto what percentage targeted drug therapy is successfull? If we Block one target, will this tumors bypass the blocked...
03 April 2011 7,833 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
