I want to study the ACE gene polymorphism in horse, but i am not able to find any primer informations related to that.
Can anyone help me in detecting the polymorphism, i am trying to find out the InDel polymorphism of ACE gene like that in humans. am not sure about the presence of same polymorphism in horse because the research on this polymorphism is concentrated to humans. Now i wanted to extend it to the feild of veterinary....
If anyone having idea about the ACE-1 gene polymorphism in horse, please help me.
How to design a new primer for detecting the polymorphism in above said gene as the location of polymorphism or even its presence are unknown???
Hello, Pramod Darvin,
You can find equine ACE primers in Table S1 in the publication:
Hamilton NA, Tammen I, Raadsma HW. Multi-species comparative analysis of the equine ACE gene identifies a highly conserved potential transcription factor binding site in intron 16. PLoS One. 2013;8(2):e55434. doi: 10.1371/journal.pone.0055434.
http://www.ncbi.nlm.nih.gov/pubmed/23408978
Hey! Promd
"Primers
Since the equine ACE coding sequence was not available at the commencement of this study, primers were selected based on the aligned cDNA sequences of the human, rabbit, rat and chicken genes"
go to the link you get useful information
http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0055434&representation=PDF
in the paper they mentioned that equine genome has similar structure to that of some known mammals, search in nucleotide and design the primers what you need.
Seems Marie also mentioned the same paper.
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