If an exogenous naphthalene-containing peptide is introduced into a cell how clearly can it be detected by u/v against the background autofluorescence of natural aromatic amino acids with the cell's proteins?
The paper linked below may be helpful to answering your questions? I am not a chemist, but this was a chemical sensor made in my lab and it can be visualized in live cells very easily. Apologies in advanced if naphthalene and naphthalimide are not remotely the same.
Article A series of naphthalimide derivatives as intra and extracell...
Many thanks for your response
I did want a naphthalene congener - I now think EDANS may be the best way to go but thanks nonetheless.
Hilmar warenius
I think it can be visualized by uv microscopy by regulating the wavelength
Hi Hilmar M Warenius you can use TD-DFT calculations for simulation and fluorescence study. gaussian software is very useful for this method!
Hi Hilmar M Warenius you can use TD-DFT calculations for simulation and fluorescence study. gaussian software is very useful for this method!
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