Name of the dye in sheaffer scrip ink?

I am dying fungi with dilution of sheaffer ink as described in the literature. Does anyone know the name of the dye used in this ink

06 July 2018 5,194 0 View

Does PLFA measure fungal spores?

Soil samples that are high in fungal spores read high in a test we have for total microbial biomass but do not correlate with PLFA results.

06 July 2018 8,016 0 View

Does the carbon burst test measure predominately anaerobic activity?

06 July 2016 7,956 3 View

Can anyone tell me how to calculate the volume of organisms on a microscopic photo?

I want to be able to estimate the volume of a mixture of soil microbes in a defined volume field such as with a hemocytometer?

03 April 2016 2,180 2 View

Do you know of a cell phone app that can read dip sticks semi-quantitatively?

11 December 2015 531 1 View

Can anyone tell me where I can get samples of soil with chloroform fumigation assay results?

I am looking to correlate results of our new test with the chloroform fumigation method.

09 October 2015 5,349 2 View

Are you looking to collaborate on validation of a new on-site method of estimating microbial biomass?

We have developed a rapid on site test for microbial biomass. We would like to correlate it to Microbial Biomass by Carbon Fumigation. I am at a Community College, can't afford this test. Looking...

08 September 2015 7,101 6 View

I am looking for a flow through device I can purchase for a research assay. Does anyone know where I can purchase?

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10 November 2014 3,130 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View