Does PLFA measure fungal spores?

Soil samples that are high in fungal spores read high in a test we have for total microbial biomass but do not correlate with PLFA results.

06 July 2018 8,016 0 View

Does the carbon burst test measure predominately anaerobic activity?

06 July 2016 7,956 3 View

Can anyone tell me how to calculate the volume of organisms on a microscopic photo?

I want to be able to estimate the volume of a mixture of soil microbes in a defined volume field such as with a hemocytometer?

03 April 2016 2,180 2 View

Do you know of a cell phone app that can read dip sticks semi-quantitatively?

11 December 2015 531 1 View

Can anyone tell me where I can get samples of soil with chloroform fumigation assay results?

I am looking to correlate results of our new test with the chloroform fumigation method.

09 October 2015 5,349 2 View

Are you looking to collaborate on validation of a new on-site method of estimating microbial biomass?

We have developed a rapid on site test for microbial biomass. We would like to correlate it to Microbial Biomass by Carbon Fumigation. I am at a Community College, can't afford this test. Looking...

08 September 2015 7,101 6 View

I am looking for a flow through device I can purchase for a research assay. Does anyone know where I can purchase?

z

10 November 2014 3,130 0 View

Does anyone know a vendor of chitin binding protein?

Looking to label fungi.

03 April 2014 6,489 1 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View