I've been working with pHILIC for Metabolomics analysis, with UHPLC+Q-Exactive. I'm now using a nanoLC (easyLC 1000 from Thermo) + Q-Exactive HF, and unfortunately pHILIC beads are not available for nano column, not even the column itself.
The advantage of using pHILIC was the high pH tolerance, allowing me to have a good coverage of metabolites (both in negative and positive).
I'm using now HILIC beads with a poor tolerance at high pH.
I am not sure that, they would be beneficial for you or not, but as far as I know,
ZIC®-HILIC HPLC Column Nano, Capillary, and Microbore Columns have a limit of pH 8 as the highest limit as a result of the PEEK sheeted fused silica tubing.
Also, I heard that Pharmafluidics will launch the HILIC phase as a micropillar array nano column, but pH ranges will probably similar to those of traditional silica-based.
Please look at the following below links which may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242784/pdf/massspectrometry-9-1-A0080.pdf
https://www.jstage.jst.go.jp/article/massspectrometry/9/1/9_A0080/_pdf/-char/en
Thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH