Does anyone know a sotfware to that helps you to mutate a gene sequence?

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How can I compute reliability taking into account repeated measures data, contemporary groups, and removing parent average effects?

I am a little bit confused about how to calculate reliability for EBV of each animal using the BLUPF90 program; taking into account repeated measures data, contemporary groups, and removing parent...

13 August 2020 4,862 5 View

When a protein is phosphorylated, how does the band look like in western blotting?

A super basic cell biology question for cell biology people. I will try to measure protein phosphorylation, and I will do western blot to see whether the phosphorylation occurs. If phosphorylation...

07 June 2020 8,995 4 View

Any equipment or technique to sterilize microbiology culture rooms?

Hi everybody I have a big contamination problem with fungi in my microalgal culture room and we have tried cleaning and disinfecting everything with thymol 5 %; we constantly clean, however the...

18 November 2019 2,331 3 View

Can some one please analyse these SEM-EDS reports to me and provide me an additional understanding to my Masters thesis?

Wanted an explanation on how I could differentiate this SEM results.

18 August 2017 491 4 View

Why do the maxima of the Q and Soret bands in phthalocyanines shift to a shorter wavelength in diffuse reflectance analysis in the solid state?

Phthalocyanines were deposited as thin films onto glass and silicon substrates.by high vacuum deposition.

02 October 2016 319 3 View

Does anybody have any evidence about the existence of mTOR autoantibodies?

One would expect that due to the protein nature of enzymes, sooner or later the mTOR signaling system would eventually develop an immune dysregulation. This opens the door to hardcore evidence to...

01 June 2014 3,439 2 View

What are the new new trends for modelling fracture in composites?

There have been a lot of failed simulations based on continuum mechanics. Last decade, a number of numerical techniques based on discontinuum representation of cracks, delamination, etc., have...

10 November 2013 7,125 4 View

What are the new new trends for modelling fracture in composites?

There have been a lot of failed simulations based on continuum mechanics. Last decade, a number of numerical techniques based on discontinuum representation of cracks, delamination, etc., have...

05 November 2013 1,704 3 View

Does anybody have any information on the deleterious effects of electromagnetic fields (cell phone radiation and Wi-Fi) on mTOR?

The declaration of cell phone telephony as a type II-B carcinogen made by the World Health Organization paves the way to all sorts of damaging interactions with human cells. In this sense, the...

12 February 2013 3,829 3 View

What is the best method for Extraction RNA From the Sperm cells????

what is the best method for Extraction RNA From the Sperm cells????

01 January 1970 569 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View