Which type of press is proper and common for making PZT parts?

What kind of press is using to make electroceramic parts, including PZT parts (hydraulic press, pneumatic press, etc.)? Furthermore, what is the reason for using this type of press? And does the...

28 December 2020 1,215 2 View

Can anyone share a protocol please for detecting presence of a hypothetical antibody against a known antigen in mice plasma?

I have hypothesized presence of some antibodies against proteins of interest to likely be present in plasma in disease. How I can quickly check this hypothesis on plasma collected from control and...

27 December 2020 9,041 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

What is the best journal for my paper?

Hi there, I am searching for the best journal suited to my paper. On the website of publishers like Springer, Elsevier,... we can use the advance search option but that is only limited to that...

17 December 2020 210 4 View

What should be the p-value for statistical analysis in a burn wound treatment study?

Hi, In our study we are going to evaluate the effect of 3 different treatments on burn wound using rat models. what should the P-value cutoff be For statistical analysis of our q-pcr output and...

05 September 2020 2,963 3 View

What are the most valid and reliable tools or simulation software to optimize building design?

Hello, I would like to know what are the most valid and reliable tools or simulation software to optimize building design with the aim of improving Indoor Environment Quality and reducing energy...

12 July 2020 7,757 9 View

HPLC column for detecting Carbonate groups?

Is there anybody who knows how can I find a suitable column for detecting a nonaqueous liquid possibly containing Carbonate groups i.e. DMC, EC, etc using HPLC chromatography methods? I don't...

30 May 2020 5,196 5 View

Can strength of the correlations using r-values be interpreted differently in different fields?

Hello, I wonder if classifications on the strength of the correlations using r-values can be different in different fields? For example, Cohen has proposed classifications for the strength of...

21 May 2020 1,848 7 View

How to interpret findings when two variables are not correlated but have linear regression?

Hello, How can I interpret my findings when X shows contribution to the regression model (X predicts a unique variance in Y), however, X is not correlated with Y? Thank you.

22 April 2020 3,007 4 View

Does anybody know some prominent books in Cognitive Linguistics?

I would like to carry out my research and need to know more about the cognitive linguistics and psycholinguistics.

16 April 2020 9,368 8 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Multiple insertions with Q5 Site-directed mutagenesis?

03 February 2021 221 2 View