Does anyone know a protocol to remove a derivative of a mini-F plasmid from an E. coli strain in order to use the resulting strain as a host?

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Are you interested to participate in this project as a postdoctoral researcher?

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Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

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Best plasmid could be used as a standard curve for AAV titration?

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Alejandro Martin

You can do plasmid curing with acridine orange or ethidium bromide. Essentially, you grow the plasmid-bearing cells on the presence of sub-inhibitory concentrations of either compound, plate and then screen the resulting colonies for plasmid loss. The process is not exactly "efficient" though; in a best-case scenario at most 5% of the screened colonies will have lost the plasmid.

Hope it helps!

Alexandra Johnson

Which strain is it?