Does anyone know a protocol to demonstrate phagocytosis of apoptotic sperm in testes of insects?

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Jeny Shklover

Try to contact Dr Eli Arama in the Weizmann institute

Henry Clemente

We produce magnetic particles to deplete apoptotic sperm. I would suggest the capture of apoptotic semen then subjecting them to 50 nm or less magnetic particles coated with dyed compounds and see if the nanoparticles are taken in by the sperm. We have done this with CHO cells and tracked the pathway of the internalized nanoparticles to the lysosome