15 Questions 14 Answers 0 Followers
Questions related from Julien Pompon
Hi, I need humanized mouse models for flavivirus infection but I do not have the skills to produce them. Do you know of a company that can do it. Thank you very much
21 February 2023 3,059 0 View
Hi all, I have beautiful amplification curves with TaqMan probe on the LC96. Probe is FAM. However, analysis with the software does not give Cq! The software has very limited options and I am...
22 January 2020 2,686 4 View
Dear all, I am planning to buy a robot to extract RNA. Any suggestions on which one is the best? Thanks
17 February 2017 1,787 4 View
Dear all, We are trying to generate primary cell lines from mosquitoes but strugggle in having them dividing. Anyone know a growth factor of insect cells from Drosophila for instance. Thank you
22 July 2016 6,904 1 View
Hello all, I would appreciate your input on which labelling to use (advantages/disadvantages) between iTraq and SILAC in cell culture thank you very much
25 February 2015 1,602 5 View
Hello, I just arrived in a new lab with an insectary that has no built-in humidity regulator. I am looking to purchase a humidifier that can regulate humidity, therefore one that can at the same...
02 October 2014 1,689 5 View
Hello, Do anyone know where I can find SILAC Schneider medium? If does not exist, is there an easy to make it myself, like adding the isotope only in medium just lacking Lysin and Arginin? Thanks
18 September 2014 6,762 3 View
I am planning an oral infection of mosquitoes with dengue virus and wonder what could be the impact of viremia (calculated in pfu here) on the resulting infection in mosquitoes. I searched the...
26 August 2014 7,768 3 View
I found 2 methods to knock down genes in mosquito larvae: chitosan-linked dsRNA feeding (Zhang et al, 2010) and rehydration driven RNAi (Lopez-Martinez et al, 2012). Both have few citations, but...
05 December 2012 6,242 13 View
I use gamma radiation to study a phenomenon, but would like to extrapolate my finding to natural setting based on the similarities between gamma- and UV- induced damages.
22 November 2012 7,422 29 View
I would like to use CypHer5 coupled with actin anti-body to study endocytosis of apoptotic sperm in mosquitoes. Does anyone have experience with CypHer 5 dye?
14 November 2012 1,749 0 View
I read that AO can stain apoptotic cells and that its emission wavelength changes when the cells are phagocytosed. However, I am surprised that there are not more people using this tool. Can...
13 November 2012 6,780 6 View
I have a protein which I think is involved in the clearance of apoptotic cells. I showed that its depletion increases the number of apoptotic cells, and that it colocalizes with apoptotic cells,...
09 November 2012 9,703 1 View
I would like to implement an assay to show in vivo that apoptotic sperm are phagocytosed in insect testes. I use TUNEL to detect apoptotic cells.
09 November 2012 9,716 2 View
Hi all, We are about to order proteins with GeneScript. Anybody has feedback to share before we move forward. I am looking for quality or any other issue that is relevant to research. Important,...
01 January 1970 8,326 3 View
