What is the difference between pS529 and pS536 for p65?

Does anybody know what the phosphorylation of different Serine residue does to NFkB p65 activation? I have an assay by Western blot detecting pS536 of p65 and a flow cytometry assay using pS529 of...

11 December 2013 3,009 8 View

CD40L antibody or Cd40L beads?

I need to stimulate B cells with CD40 ligand. Which kind of stimulation will be the most efficient: beads or antibody?

08 September 2013 8,966 13 View

RNA seq analysis

Hello, I have the RNA seq results (expressed as RPKM) of 8 samples: 1 set of control and 1 set of knock down for my gene of interest, all of the treated with 4 different cytokines. Does anyone...

01 February 2012 8,375 3 View

Extract the protein and the RNA from a single dish

I would need to extract the protein and the RNA from a single dish as my sample are quite precious, so I could use a limited amout of material. Is there a protocol or a kit that would allow that...

10 November 2011 3,352 8 View

First detection re-appears with a different pattern

Hi all, I have detected a protein at 63kDa Attached Image: Scan 1.jpg and re-used the membrane to detect the loading control at 42 kDa Attached Image: Scan 2.jpg. But doing so, the first protein...

09 October 2011 5,864 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View