Hi all,

I have detected a protein at 63kDa Attached Image: Scan 1.jpg and re-used the membrane to detect the loading control at 42 kDa Attached Image: Scan 2.jpg.

But doing so, the first protein was detected again (which is normal as it is the same secondary antibody), my problem is that the pattern was different, I have bands where there was nothing previously.

So I don't know which of the detection is the "real" one.

Any suggestion about how I could know which one is "real"?

Thanks