Sylviane Lambert

6 Questions 21 Answers 0 Followers

Questions related from Sylviane Lambert

What is the difference between pS529 and pS536 for p65?

Does anybody know what the phosphorylation of different Serine residue does to NFkB p65 activation? I have an assay by Western blot detecting pS536 of p65 and a flow cytometry assay using pS529 of...

12 December 2013 3,105 8 View

CD40L antibody or Cd40L beads?

I need to stimulate B cells with CD40 ligand. Which kind of stimulation will be the most efficient: beads or antibody?

09 September 2013 9,055 13 View

Does anyone know a protocol for flow cytometry with keratinocyte?

Would someone have a good protocol to do flow cytometry with keratinocytes. They have tendency to aggregate once detached. Any ideas or good references?

01 January 2013 6,713 2 View

RNA seq analysis

Hello, I have the RNA seq results (expressed as RPKM) of 8 samples: 1 set of control and 1 set of knock down for my gene of interest, all of the treated with 4 different cytokines. Does anyone...

02 February 2012 8,478 3 View

Extract the protein and the RNA from a single dish

I would need to extract the protein and the RNA from a single dish as my sample are quite precious, so I could use a limited amout of material. Is there a protocol or a kit that would allow that...

11 November 2011 3,441 8 View

First detection re-appears with a different pattern

Hi all, I have detected a protein at 63kDa Attached Image: Scan 1.jpg and re-used the membrane to detect the loading control at 42 kDa Attached Image: Scan 2.jpg. But doing so, the first protein...

10 October 2011 5,955 2 View