Does anyone know a product that will allow flow cytometry directly on 'blood' samples?

Benedict Lyle Phillips @Benedict_Phillips

10 October 2018 1 1K Report

I have samples containing lots of packed red blood cells and small numbers of leukocytes.

Does anyone know a product that will allow flow cytometry directly on this sample without having to separate cells using a ficoll gradient?

Thomas Schmitt

Simple suggestion, use a centrifuge and PBS or another suitable buffer.

Just centrifuge the cells, wash the pellet 1-3 times with PBS and then prepare them for you flow cytometry experiment. This should work pretty okay, without too much loss of cells if you work carefully.

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