Does anyone know a low molecular weight agarose gel?

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Why are functional gene results (nosZ, amoA) compared to 16S gene?

I am working on the effect of pH on functional genes in the nitrogen cycle (amoA, nosZ..). Some of the papers i have read present results based on the ratio between functional gene (like nosZ) and...

27 December 2020 1,625 6 View

Why would gel electrophoresis need to be run for 16 hours at 2-8 deg C? Is there an alternative protocol that takes less time or works at room temp?

I am trying to separate and analyze macromolecules (DNA) using horizontal gel electrophoresis. Protocol involves 1X TAE buffer, 10X denaturing buffer, 1X running buffer, 1% agarose gel, and 2X...

18 November 2020 667 2 View

Any tips and tricks to prevent bubbles from being trapped in polymer film during membrane formation via immersion precipitation?

05 July 2017 6,398 6 View

Why does the level of phenobarbital spiked in blood appear to be lower than the actual spiked amount when being screened by ELISA?

5ppb of phenobarbital have been spiked into blank blood. This have been replicated by 10 times. However, ELISA results show that all 10 of the replicates contains LESS than 5ppb (average of...

14 July 2013 963 1 View

Can anyone help me with a home made OGD?

I'm hoping to set up some oxygen glucose deprivation experiments using cultured cells but only have a CO2/air incubator. I was wondering whether there was any way of home rigging a small nitrogen...

06 June 2013 2,650 2 View

Will the formation of semicarbazone from an unknown (suspected to be a ketone) be long, or has it been falsely analysed?

In an undergraduate organic experiment, we are required to identify the functional group present via solubility tests (with H2O, HCl, NaOH and NaHCO3) and Classification Tests (Brady's, Tollen's,...

10 March 2013 3,007 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View