I will be conducting research on potato drought tolerance. I would like to know the best time to begin the drought simulation on the potato plants (I want to make sure the potatoes establish themselves well before I begin the drought simulation).
I am working on the effect of pH on functional genes in the nitrogen cycle (amoA, nosZ..). Some of the papers i have read present results based on the ratio between functional gene (like nosZ) and...
27 December 2020 1,625 6 View
I am trying to separate and analyze macromolecules (DNA) using horizontal gel electrophoresis. Protocol involves 1X TAE buffer, 10X denaturing buffer, 1X running buffer, 1% agarose gel, and 2X...
18 November 2020 667 2 View
05 July 2017 6,398 6 View
I need a trade name for any low molecular weight agarose gel that can dissolve in water. My cDNA is giving me my band of interest (which is faint) and a smear on gel and I want to cut out my band...
23 April 2014 8,122 2 View
5ppb of phenobarbital have been spiked into blank blood. This have been replicated by 10 times. However, ELISA results show that all 10 of the replicates contains LESS than 5ppb (average of...
14 July 2013 963 1 View
I'm hoping to set up some oxygen glucose deprivation experiments using cultured cells but only have a CO2/air incubator. I was wondering whether there was any way of home rigging a small nitrogen...
06 June 2013 2,650 2 View
In an undergraduate organic experiment, we are required to identify the functional group present via solubility tests (with H2O, HCl, NaOH and NaHCO3) and Classification Tests (Brady's, Tollen's,...
10 March 2013 3,007 6 View
I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...
23 February 2021 1,616 5 View
How much time is required from germination of pollen grain on stigma to fertilization in Solanaceous crops (chilli, tomato, brinjal)?
26 December 2020 887 5 View
For my research it's needed to measure the concentration of the following sugars present in a potato tuber: sucrose, fructose and glucose. Is there a reliable method to measure these with a...
07 September 2020 5,718 4 View
Presently about forty food crops are in common use in the world over. Amongst these four crops namely wheat, rice, maize and potatoes dominate the scene. However, I want to know which food crop is...
06 August 2020 1,755 19 View
Hello, this is yet another urgent question for my assignment: Does the genus Capsicum contain solanine in their stem like other nightshades do? Thank you for your answers in advance!
03 June 2020 3,827 11 View
I have non embriogenic callus from Tamarillo (Solanum betaceum) in liquid culture medium, and i would like to quantify the cells, but they form aggregates. Any advices?
04 February 2020 4,328 4 View
01 February 2020 1,348 3 View
Perhaps people who work with protein extraction could share some ideas and tips on the best methods to extract patatin, chemicals used, quantification methods? What is working, what is not? I...
09 January 2020 2,087 3 View
31 December 2019 1,896 3 View
I am finding trouble in isolation of Phytophthora infestans from potato leaves in PDA? What might be the reason? Which could be the best media?
31 December 2019 2,563 9 View