Why are functional gene results (nosZ, amoA) compared to 16S gene?

I am working on the effect of pH on functional genes in the nitrogen cycle (amoA, nosZ..). Some of the papers i have read present results based on the ratio between functional gene (like nosZ) and...

27 December 2020 1,625 6 View

Why would gel electrophoresis need to be run for 16 hours at 2-8 deg C? Is there an alternative protocol that takes less time or works at room temp?

I am trying to separate and analyze macromolecules (DNA) using horizontal gel electrophoresis. Protocol involves 1X TAE buffer, 10X denaturing buffer, 1X running buffer, 1% agarose gel, and 2X...

18 November 2020 667 2 View

Any tips and tricks to prevent bubbles from being trapped in polymer film during membrane formation via immersion precipitation?

05 July 2017 6,398 6 View

Does anyone know a low molecular weight agarose gel?

I need a trade name for any low molecular weight agarose gel that can dissolve in water. My cDNA is giving me my band of interest (which is faint) and a smear on gel and I want to cut out my band...

23 April 2014 8,122 2 View

Why does the level of phenobarbital spiked in blood appear to be lower than the actual spiked amount when being screened by ELISA?

5ppb of phenobarbital have been spiked into blank blood. This have been replicated by 10 times. However, ELISA results show that all 10 of the replicates contains LESS than 5ppb (average of...

14 July 2013 963 1 View

Can anyone help me with a home made OGD?

I'm hoping to set up some oxygen glucose deprivation experiments using cultured cells but only have a CO2/air incubator. I was wondering whether there was any way of home rigging a small nitrogen...

06 June 2013 2,650 2 View

Will the formation of semicarbazone from an unknown (suspected to be a ketone) be long, or has it been falsely analysed?

In an undergraduate organic experiment, we are required to identify the functional group present via solubility tests (with H2O, HCl, NaOH and NaHCO3) and Classification Tests (Brady's, Tollen's,...

10 March 2013 3,007 6 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View