What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

Any instances of when an antibody will bind better if left at RT for days?

Can anyone make sense of this: an antibody was left out for 7 days on the bench at RT and binds better to a metalloprotein than if the antibody was taken out the day of the experiment?

15 April 2020 6,330 3 View

How do I calculate solubility of a compound using a turbidity assay?

I'm planning to measure solubility of my compound in PBS buffer by measuring the turbidity of serially diluted solutions in a microplate reader at 600 nm. Can anyone please inform me how to...

23 November 2019 3,333 3 View

Is anyone using Winepiscope 2.0 epidemiological veterinary medicine software for risk analysis?

The website version is already available but it is time consuming to feed manually. The software is in need very urgent. Please let me know ASAP.

23 October 2019 250 2 View

How to sort out a barcode mix up dilemma in a RNAseq experiment?

Hi there, So I submitted 20 RNA samples to a sequencing facility for bulk RNAseq. I got the results back and I actually suspect there might be a mix up of samples. Out of the desperation, I ran...

04 October 2019 2,801 4 View

Networking - Can you all help me network with former psychologists or counselors from corrections/juvenile detention centers, etc?

I am hoping to find connections with former psychologists and counselor who have worked with correctional populations, addictions, etc in hopes of learning from them about their experiences, any...

30 August 2019 10,008 2 View

What are some reliable methods for validating antibodies?

I want to do a pre-absorption technique, but I'm having trouble finding pure proteins of the following: ASIC 2 NFP (2F11) S46 S100P If anyone knows a good site I can purchase pure proteins or if...

16 August 2019 483 3 View

Has anyone any idea about various softwares that can analyze various aspects of large number of texts?

Is there any one who can give names of softwares that can analyze various aspects of texts?

24 February 2019 7,687 15 View

Can anyone suggest a viral vector encoding for firefly luciferase for transfection into human leukemia cell line MV4-11?

I am currently working on developing a bioluminescent mouse model for investigating the anti-leukemic activity of our test compounds. For that purpose I wish to conduct the lentiviral transfection...

23 January 2019 7,572 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View