How to determine the orders of High pass filter for ECG signal ( cutoff frequency = 0.033 Hz so near 0 Hz) ?

I'm trying to theoretically design a active High-Pass Filter for ECG signal with cutoff frequency Fc = 0.003 Hz...it's too close to 0 Hz and stop band frequency must be greater than 0 Hz . So...

18 November 2020 9,089 1 View

Plaxis V20 water load apply error in analyses?

Recently I have been working in some embankment project and plaxis V20 is the FEM software I am using to calculate. But by the time I am working, I figured out the water load sometimes applied to...

27 October 2020 7,835 1 View

How to interpret when moderating effect is significant but direct effects are not?

Hello everybody, I am currently conducting a quantitative research, using linear regression. My 1st hypothesis says that A has a positive effect and my 2nd hypothesis says that B has a positive...

03 February 2020 3,933 5 View

Why does the PCR stops working suddenly?

Hi all, My PCR to amplify 9kb fragment of barley was working well and give clear bands (9kb and one unspecific band about 1 kb). All the PCR conditions have been optimized, and they worked...

14 March 2019 2,458 12 View

Dear all, I would like to ask how to melt Lithium?

I put 1 gram of Li foil on ceramic pot and put they in hot plate in glove box, the temperature was maintained at 350-C for more than 2 hours but it is not work. Is there any pre-treatment for this...

11 October 2017 1,459 6 View

Can I ship cells for RNA and protein extraction overseas on dry ice?

21 November 2016 5,754 2 View

Can someone suggest me how to homogenize Artemia nauplii ?

I want to investigate microflora inside of Artemia nauplii, so I want to break the body of Artemia nauplii. what kind of homogenizer machine can I use? Or which method can I use? Thank you so much?

10 November 2016 9,106 3 View

Is there any virus member in Flaviviridae family which dose not create the replication vesicles inside ER membrane during its replication?

09 September 2015 9,860 3 View

Could someone help me answer a question about SDS - PAGE?

I did a one dimensional SDS - PAGE. Stacking gel is 5% and Separating Gel is 12%,however, they didn't polimerize. I tried to use progressive concentration of Acrylamide from 12% to 36%, and at 36%...

18 July 2015 3,381 18 View

Can someone help me in identifying this species of genus Schistura in Vietnam?

ichthyologist, freshwater fish, taxonomy

29 June 2015 8,267 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View