The samples are from marine and wild animals with bacterial, fungal and parasitic agents. Some cases are first reported in the world. My intention is to join histopathology with the identification of these infectious agents.
Hi Jerdy, after fixation and H&E staining you can only say you have a contamination of bacterias, fungus or parasites but not exactly what kind of.
You can verify your result by PAS staining. In this staining it is easier to see the different forms of fungus or parasites. A differentiation of subtypes is not possible.
Dear Dr Jean-Martin,
Formaldehyde-fixed tissue samples (kidney, intestine, liver, lung). I know it's possible to extract DNA from formalin-fixed samples.
OK, so you have (or will have) genomic data from the samples, and want to correlate that with histopathology ? Standard histopathologic methods can only very broadly indicate the presence of agents, eg. for bacteria you can confirm presence of gram-negative or gram-positive, for fungi you can confirm with a PAS or a silver stain the presence of the agents and look at their morphology, etc... But you will not be able to identify down to the species or even genus in most cases. For this you need immunohistochemical stains, which require having a specific antibody for each of the agents you want to confirm. Such antibodies might be available for known agents, but for novel ones it will not be possible.
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