Does anyone here use chitinase, beta-glucanase and chitosanase as a preventive reagent for fungal and/or yeast in cell culture?
I found someone in reseachgate recommended this over fungizone.
In my personal experience, antifungal properties of beta-glucanases
are a way less robust than what it appears like from the literature.
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
I am now developing a python module for ms2 database searching, would like to realize a function that similar to what Xcalibur did, choose multiple mass spectra and get an averaged spectra. But...
22 July 2024 3,975 1 View
I am using gelatin to model mechanical properties of brain tissues in-vitro. Do you have any suggested concentration ? Thank you so much.
19 July 2024 3,095 0 View
It is best to have the route distance between ports, the size of the shipping alliance market, direct service, the number of ships owned by the shipping alliance and the congestion of ships in the...
06 July 2024 845 1 View
Dear professors and colleagues, hello!Recently, while studying the CDP model, I have read the manuscripts of various experts and have gained a lot, but I still have some questions.What I...
10 June 2024 1,906 1 View
Hi~ We are making different Cas9 plasmids such as regular spCas9, base editing and prime editing under different promoter such as CMV and CAG. During selection of colonies, we always get a good...
22 May 2024 7,336 0 View
When I calculated Kd, I found that although the concentrations were set to satisfy the type of binding used to determine the differential spectrum, Kd was unstable if the two high concentrations...
14 May 2024 399 0 View
Kindly greetings, In a conference I attended before, the peer reviewers believed that the flexural strength, compressive strength, and Young's modulus in the submitted manuscript could not fully...
08 May 2024 7,171 3 View
Meissl P (1962) Die innere Genauigkeit eines Punkthaufens. Österreichische Zeitschrift für Vermessungswesen 50, Nr. 5, S. 159–165, Nr. 6, S, pp 186–194 Meissl P (1965) Uber die innere Genauigheit...
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Methods are based on Chel-Guerrero and colleagues'Phytochemical Profile, Toxicity, and Pharmacological Potenti... . We use a 1M Tris buffer with pH of 6.8, with Bovine Serum Albumin as the...
25 April 2024 8,802 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View