Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

Why are we measuring gel content of NBR latex? Does gel content related to the amount of cross-linked in a polymer?

Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...

07 February 2021 605 4 View

How to delete a copy of a paper, and how to delete a co-author's name?

I mistakenly uploaded a copy of the same paper and entered my alternative name. How to delete them out? (I couldn't navigate to the right buttons to do it. So from where to where is what I don't...

07 January 2021 7,694 6 View

What effect will different solvents have on the ATRP reaction?

I want to do an ATRP reaction, synthesize an ATRP initiator, use DMF as a solvent, and mix DMF, BIBB and TEA to turn red. Why does it turn red? Xylene has been used as a solvent before, and there...

04 January 2021 8,034 3 View

Species identification.Who can tell me the scientific names of these Gastropods?

Species identification. I've been doing research on communities of marine macrobenthos recently, but some of them can't be identified, which hinders my research. Who can help me identify these...

14 December 2020 8,357 3 View

Cell shrinkage during ROS assay(H2DCFDA)?

Hi! I'm Yu, studying about health of MSC. These days, I'm doing ROS assay with H2DCFDA which requires 5~30 minutes of staining with PBS or HEPES or HBSS. In our lab, We use PBS w/o Mg/Ca for...

13 December 2020 8,813 9 View

Can I fix the cells after H2DCFDA assay for FACS analysis?

Hi ! I'm Yu, and planning to analyze ROS assay with H2DCFDA(Thermo) on MSCs. A lot of protocols instruct that treat H2DCFDA at specific concentration for 5~30 min, 20~30 min of recovery time...

10 December 2020 973 1 View

How to reconstruct a Lamb wave by a series of pulses superposed?

Lamb wave is useful for solid structure health monitoring, and it consists many modes. Unlike ultrasonic bulk wave, when given a pulse excitation (for example, 5-cycle sinusoidal pulse), only...

08 December 2020 5,440 8 View

How to interpret the significant effect of covariates when conducting a Two-way ANCOVA?

06 December 2020 8,560 4 View

Why am I unable to coat oleate-capped NaCeF4:Gd,Tb nanoparticles with mesoporous silica shell?

My oleate-capped NaCeF4:Gd,Tb NPs have been synthesized via the thermal decomposition method. 1mmol of NPs has been dispersed in 20 ml of cyclohexane. I have tried dissolving 1 ml of NP in...

01 December 2020 4,713 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View