Ming Chuan Yu

10 Questions 39 Answers 0 Followers

Questions related from Ming Chuan Yu

Has anyone here experience with culturing Breast cancer stem cells (Parental)?

I would like to know your experience on culturing Human breast cancer stem cells (Parental) Thanks.

11 August 2017 5,035 3 View

Do we need to do statistical analysis for miRNA expression that showed in fold change?

I get fold change of miRNAs expression after running qPCR. I would like to know whether I need to do statistical analysis for those fold change or not? in fold change, if expression level is above...

18 June 2017 682 4 View

How many times do I have to passage in order to confirm that Primary Breast Cancer Cells become an immortalized breast cancer cell line?

I heard that we have to passage until 50 passages in order to claim that the primary breast cancer cells become an immortalized cell line. Is this correct? Where can I find this information? I...

21 November 2016 2,383 5 View

What is the best way to prove primary breast cancer epithelial cells in cultured flasks are tumor cells?

I am currently culturing primary breast cancer epithelial cells from human breast cancer patient. It has been about 3 to 4 months and now epithelial cells are growing well and no more fibroblast...

10 October 2016 8,872 3 View

How to remove debris after tissue dissociation for stem cell culture?

I am facing an issue of debris after dissociation. These debris are mixed with dissociated cells. As I am using serum free and ultra low attachment flask, it is really hard to remove them. If you...

13 July 2016 6,467 2 View

Can anyone share Graphpad Prism software but not trial version?

I would like to find out whether anyone can share graphpad prism software. I would like to have it. Thanks. Trial version just can be used for a month only. Thanks,

16 May 2016 8,509 3 View

What type of flasks are suitable for culturing breast cancer stem cells?

Can we use just normal flasks that are routinely used for cell cultures? I realized that it is the best if we don't let cells to attach the surface when culturing stem cells, especially breast...

02 April 2016 4,196 2 View

How should I culture H9C2 cells successfully?

Is anyone here culturing H9C2 cells? My H9C2 cells growth rate is very slow and I am not sure what is the problem with this. I am using DMEM with 10% FBS and 1% sodium pyruvate and Passage 24 of...

22 December 2015 916 10 View

Does anyone have advice on sealing cryovial and storing in liquid Nitrogen tank?

Hi All, I would like to check whether commercially available cryovials with silicone gasket, internal thread are safe enough to store in liquid phase of nitrogen tank or not. I see all labs are...

25 November 2015 1,230 10 View

Does anyone here use chitinase, beta-glucanase and chitosanase?

Does anyone here use chitinase, beta-glucanase and chitosanase as a preventive reagent for fungal and/or yeast in cell culture? I found someone in reseachgate recommended this over fungizone.

01 January 1970 6,031 1 View