Does anyone have the experience with mouse DRG neuron culture (for western blot analyses)?

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Is Esp3I more efficient at digesting supercoiled DNA than BsmBI?

I've been working on optimizing a restriction digest of a plasmid using BsmBI/Esp3I. When running my uncut plasmid on a gel, there is a lot of supercoiled plasmid. I've been playing around with...

27 January 2022 4,608 0 View

Having all research questions as mixed-methods questions in a mixed-methods study: what to cite as support?

Hi there, I am wondering what work I can cite in support of having several research questions in a mixed-methods study that are ALL mixed-methods in nature? I have 5 research questions, some are...

09 August 2018 3,035 4 View

Validations: can an orthogonal confirmation of specificity for a GMP product be performed in a non-GMP lab?

I need to confirm the specificity of the peaks in my HPLC. This validation is for the commercial analytical test for a protein-based pharmaceutical. One of our researchers working out of the USDA...

08 June 2017 3,091 2 View

Does anyone know of a company that sells Poly-D-Lysine or Poly-L-Lysine labeled with a flourescent dye other than Fitc?

I can not find a company that sells PDL/PLL labeled with anything other than Fitc. Preferably PDL/PLL labeled with Tritc or Cy5?

23 April 2017 7,131 1 View

In TBI, we see a variety of forces such as compressive, shearing, and tensile. What would be the axons response to shearing forces vs stretch/tensile?

No paper gives me an answer as to why solely using an axonal stretch model to study the effects of TBI is a good idea or why they chose it over studying shearing forces. Can anyone explain what...

11 April 2017 456 2 View

In Traumatic Brain Injury, what difference in neural response would we see in axonal stretch injury vs. axonal shearing injury?

No paper seems to explain the difference in neural response to these different types of forces imposed on neurons. Would you see more secondary damage in one of them or would they result in very...

11 April 2017 763 2 View

What are the reference values for protein and albumin content in bronchoalveolar fluid?

Observing a very frothy white layer on a bronchoalveolar fluid obtained with a very good yield (155/200ml) prompted me to measure both the albumin and protein content of this fluid. The beer-like...

25 February 2015 8,300 6 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View