I've been working on optimizing a restriction digest of a plasmid using BsmBI/Esp3I. When running my uncut plasmid on a gel, there is a lot of supercoiled plasmid. I've been playing around with different lengths of digest time, different amounts of BsmBI, as well as spiking in additional enzyme during the digest and digesting further to hopefully cut the supercoiled plasmid. I have done a few digestions to compare Esp3I with BsmBI, and my digests with Esp3I have looked a lot cleaner and digest more DNA to completion. I was wondering if anyone else has experienced better digestion of supercoiled DNA with Esp3I than BsmBI as I haven't been able to find anyone online talking about this.