Non motile black dots in animal cell culture, tightly adhered to the cells along the cell periphery. Is it a contamination? How to get rid of them?

I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...

18 January 2021 5,151 1 View

Best flow cytometry markers for Thp1-derived M1 and M2 macrophages?

Hi everybody, I am trying to polarize and differentiate thp1 cells to M1 and M2 macrophages. There are lots of different protocols, so I've been trying different incubation times and...

02 November 2020 8,554 3 View

Have you ever seen two peaks after preparation small RNA library for NGS?

Hi, I have problem with my libraries, I use ClenTag Small RNA Library Preparation Kit by Trilink. The material is RNA from plasma cells. I have already done this two years ago and everythink...

25 August 2020 8,459 1 View

What are the key things to write and publish a paper in a good indexed journal?

The major spices and ingredients to add flavour in a paper for a journal in the domain of Computer Vision and Machine Learning.

27 June 2020 7,967 10 View

How to decide that a work is suitable for a conference or a journal in the field of Computer Science & Engineering?

What are the deciding factors to submit a paper in conference or journal.

21 June 2020 1,604 8 View

Whether Lambert Academic Publishing (LAP) provide printed copies to author? Do authors need paymet or sharing bank setails?

Hello Everyone !! Does anyone have any experience in publishing through Lambert Academic Publishing (LAP)? Recently I got a request mail from them regarding publication. I want to know about the...

25 April 2020 2,651 2 View

How can I write the .rpy and .jnl file in Abaqus cae for windows?

Hello :) I have the Abaqus CAE student version for Windows recently. My problem is: Abaqus does not write the .jnl and .rpy files automatically during a session as I am used to from the Linux...

30 March 2020 2,579 4 View

HSV1 in human tissue - dangerous after the fixation with PFA?

Hi, we are wondering about the HSV1 virus in human tissue. We want to perform in situ sequencing with this tissue, but we are not experienced with the virus-infected samples. Is it possible that...

14 October 2019 8,969 1 View

What details should be followed in MEGA-X for the Multiple Sequence Alignment of 16S data?

I have some 16S RNA sequence with which I want to do the phylogenetic analysis, so Initially I did BLAST against SILVA db and NACBI 16S db and went for MSA in MEGA-X using Muscle and Clustal both...

21 July 2019 9,320 3 View

While using R.E.D tools for charge calculation how to use Gaussian in the place of GAMESS? I have the file.p2n file and file.log ??

I have obtained the file.log after geometry optimisation done using GAUSSIAN and I do have the file.p2n after running Ante_RED-1.5.pl then I am getting an error of not having GAMESS while I wanna...

04 April 2019 4,228 1 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View