Does anyone have recommendations regarding the adaptation of HEK293 adherent cells to suspension serum-free culture ?

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Liyuan Zhang

did you find a good protocol? if not, you can check this

https://www.xell.ag/wp-content/uploads/Adaptation-of-mammalian-cell-lines-to-serum-free-suspension-culture.docx.pdf

Claire Favre-Félix Did you find a good protocol? please ignore my reply above. i tried that one, it doesn't work well.