Are there any cell lines and/or tissues that do NOT exhibit P-bodies?

02 March 2017 1,486 3 View

Is there really only one (protein) RNA ligase in humans?

04 May 2016 4,634 2 View

Can anyone advise me on the behaviour of nucleolar proteins in the Mendez/Stillman chromatin fractionation assay?

Hi all, I am currently investigating a protein that shows both nucleolar and nucleoplasmic staining, and one of the analyses I would like to conduct is to test whether the nucleoplasmic fraction...

31 December 2015 4,857 3 View

Does anyone know of a commercially available E2F1 phosphoS31 (ATM/ATR site) antibody?

We are hoping to monitor phosphorylation of E2F1 by ATM/ATR at Ser31 by western blotting. However, while this modification has has been widely reported in the literature, I have not been able to...

10 November 2015 4,902 0 View

What are the advantages of tBHP over H2O2 for inducing ROS in cell lines?

My lab is currently conducting assays on the sensitivity to ROS of a couple of cell lines, and we are looking at the different agents used to induce ROS. I've heard that tBHP (tert-Butyl...

04 May 2014 7,863 4 View

Where can I find data on the total number of academics and/or research students per country?

I have developed a database of members of our organisation; these members are drawn from about 150 countries around the world and are mostly from academia or para-academic professions (e.g....

08 September 2013 6,550 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View