My lab is currently conducting assays on the sensitivity to ROS of a couple of cell lines, and we are looking at the different agents used to induce ROS. I've heard that tBHP (tert-Butyl hydroperoxide) is a better choice than H2O2, but can't find a lot of data as to why (beyond one paper showing a lack of DNA damage in tBHP- vs. H2O2-treated cells). Would anyone be able to shed some light on the advantages of tBHP over H2O2?
tert-butylhydroperoxide (tBu-OOH) forms a more stable radical species than the corresponding HOOH homologue. However, it is a more "bulky" substrate (due to the tert-butyl side chain) than H202. As a result, it shows some differences in substrate reactivity to certain types of catalases and hydroperoxidases.
A very high level explanation - hope that helps??
tert-butylhydroperoxide (tBu-OOH) forms a more stable radical species than the corresponding HOOH homologue. However, it is a more "bulky" substrate (due to the tert-butyl side chain) than H202. As a result, it shows some differences in substrate reactivity to certain types of catalases and hydroperoxidases.
A very high level explanation - hope that helps??
Hi, Daniel,
hhere is a quite good explanation: http://ntp.niehs.nih.gov/ntp/htdocs/chem_background/exsumpdf/butylhydroperoxide_508.pdf
In addition, tBu-OOH is much more stable to compare with traditional H2O2.
Howvere, H2O2 is also good under certain conditions
Thanks Wayne and Taras, that's really useful! tBHP it is, I think.
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